| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.153462 |
| Chromosome: | chromosome 12 |
| Location: | 1633324 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486208 | Found in Cr and Volvox but not strongly conserved elsewhere | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCACTATCCAGCCGCCGTCTACCAGCTA |
| Internal bar code: | AAAGATGGCGAGTGAGCCGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 70 |
| LEAP-Seq percent confirming: | 57.1429 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGGCAAGCTGTTGTGTTA |
| Suggested primer 2: | CTGCAACCACCTACCCCTTA |