| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.153463 |
| Chromosome: | chromosome 4 |
| Location: | 1719349 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g212500 | CGL42 | (1 of 6) PF09353 - Domain of unknown function (DUF1995) (DUF1995); Conserved in the Green Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGATGGTTTGATGGCCTGTTGAAGTGAG |
| Internal bar code: | CGACTGGGTTATCATTCTTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 538 |
| LEAP-Seq percent confirming: | 99.7044 |
| LEAP-Seq n confirming: | 4385 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTGGAAACCCATTACCG |
| Suggested primer 2: | CGCATTAGGCTAGCTGTTCC |