Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.153482 |
Chromosome: | chromosome 13 |
Location: | 3252211 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g585900 | LKHA4,LTA4 | (1 of 1) 3.3.2.6 - Leukotriene-A(4) hydrolase / LTA-4 hydrolase; Leukotriene A-4 hydrolase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGTCATAACAGGCACCCCTTTAAAACAA |
Internal bar code: | GGCCCGCTCTCGGATTTTCTAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 223 |
LEAP-Seq percent confirming: | 99.4545 |
LEAP-Seq n confirming: | 1094 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCACGACACCTTTTCATCCT |
Suggested primer 2: | ACACAGCACTTCCGGATTTC |