Insertion junction: LMJ.RY0402.153503_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.153503
Chromosome:chromosome 6
Location:3463084
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g278095 (1 of 1) IPR016024//IPR027417 - Armadillo-type fold // P-loop containing nucleoside triphosphate hydrolase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCGCCAAGAAGATCCGCGACTCGGTCCT
Internal bar code:CGCTTTCCTTAGGCTTGACTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:572
LEAP-Seq percent confirming:98.7414
LEAP-Seq n confirming:863
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TTGACTGGAAGCAGTTGTCG
Suggested primer 2:CATGCCATCACCTTCATCTG