| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.153504 |
| Chromosome: | chromosome 6 |
| Location: | 5093115 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280800 | (1 of 1) K11291 - nuclear autoantigenic sperm protein (NASP) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCTATCATACTGCCAGCATTTGGCTGAA |
| Internal bar code: | TAAGCGGCCCGCATCCGCACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 435 |
| LEAP-Seq percent confirming: | 87.4441 |
| LEAP-Seq n confirming: | 2347 |
| LEAP-Seq n nonconfirming: | 337 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCCACATCACCACACTTC |
| Suggested primer 2: | GCTTTCACAGCACGTTCAGA |