Insertion junction: LMJ.RY0402.153517_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.153517
Chromosome:chromosome 4
Location:1495493
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g213904 Mitogen-Activated Protein Kinase 3; (1 of 1) K02208 - cyclin-dependent kinase 8/11 [EC:2.7.11.22 2.7.11.23] (CDK8_11) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGATGCCCAGTAGCAAACACTTCCACAC
Internal bar code:CCGTGGACCGTCACTGGCGACC

Confirmation - LEAP-Seq

LEAP-Seq distance:303
LEAP-Seq percent confirming:99.4152
LEAP-Seq n confirming:170
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CACACACACACACACCTGGA
Suggested primer 2:AGGAAATACTTGGTGGCACG