Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.153517 |
Chromosome: | chromosome 4 |
Location: | 1495501 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre04.g213904 | Mitogen-Activated Protein Kinase 3; (1 of 1) K02208 - cyclin-dependent kinase 8/11 [EC:2.7.11.22 2.7.11.23] (CDK8_11) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATCAGCAGCATGCGAGTAGCTGACTGGG |
Internal bar code: | TCTTGGTAAGGCTCGAAGGGAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 413 |
LEAP-Seq percent confirming: | 98.7844 |
LEAP-Seq n confirming: | 2438 |
LEAP-Seq n nonconfirming: | 30 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACACACACACACACCTGGA |
Suggested primer 2: | AGGAAATACTTGGTGGCACG |