| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.153524 |
| Chromosome: | chromosome 8 |
| Location: | 3188651 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g373421 | 5'UTR|CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGGTCGGTGAGTTCCTTCAACTGCGCAG |
| Internal bar code: | TAGCGGGACGTCCCGAGTCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 292 |
| LEAP-Seq percent confirming: | 98.1627 |
| LEAP-Seq n confirming: | 374 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCTTCAAAGGTCCACAGG |
| Suggested primer 2: | GCCTGTACACCAGCACCTTT |