Insertion junction: LMJ.RY0402.153527_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.153527
Chromosome:chromosome 3
Location:8127767
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g200750 DMC1 Putative DNA methylase; (1 of 1) IPR027417//IPR029063 - P-loop containing nucleoside triphosphate hydrolase // S-adenosyl-L-methionine-dependent methyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCTCGGGGGCATCCGCCCGCCTGACGGC
Internal bar code:GCCCGCATTCCCGAATCAGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:906
LEAP-Seq percent confirming:97.8129
LEAP-Seq n confirming:2102
LEAP-Seq n nonconfirming:47
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CGCATCTGAGTCGTCCACTA
Suggested primer 2:ATAGTGTTCCGTTGCTGGCT