| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.153534 |
| Chromosome: | chromosome 8 |
| Location: | 4941827 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g385400 | (1 of 3) 3.5.1.1 - Asparaginase / L-asparagine amidohydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGTATCAGGGCTCGGCGATTGCCGGTGC |
| Internal bar code: | CGGAGAGGCCTCGGGATACCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1133 |
| LEAP-Seq percent confirming: | 99.3237 |
| LEAP-Seq n confirming: | 6903 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGTGCGGACTGCAATGAC |
| Suggested primer 2: | GACACTTGGAGCCGAGTAGG |