| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.153599 |
| Chromosome: | chromosome 13 |
| Location: | 4045302 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g591250 | EZY20 | (1 of 726) IPR011009 - Protein kinase-like domain; Early zygote expressed protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTAGAAACGGTATTACGACTGCGTTCGT |
| Internal bar code: | TTATCAATTTAACCGTATCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1064 |
| LEAP-Seq percent confirming: | 99.6457 |
| LEAP-Seq n confirming: | 1125 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTCCAGGACTCCATCAT |
| Suggested primer 2: | TTTGGCTTGGGTTGGTTTAG |