| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.153603 |
| Chromosome: | chromosome 9 |
| Location: | 2760390 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389200 | UMM8 | UbiE/COQ5 methyltransferase family protein; (1 of 1) 2.1.1.288 - Aklanonic acid methyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCCACCTTCCACGCACCCATGGGACGGA |
| Internal bar code: | GGGGGTATGCGTCATGTGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 947 |
| LEAP-Seq percent confirming: | 99.2348 |
| LEAP-Seq n confirming: | 2075 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGCCTAAGAGGCGCAGTA |
| Suggested primer 2: | CAACAACTCGGGTGCCTATT |