Insertion junction: LMJ.RY0402.153621_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.153621
Chromosome:chromosome 16
Location:1837593
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g655450 (1 of 1) PF14510 - ABC-transporter extracellular N-terminal (ABC_trans_N) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAGGGCCGGGTTTAGGTAGCCCAACTAGG
Internal bar code:GGATCGGGACACAGGGGTTAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:781
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCAAGCGAGCTAGTCCAATC
Suggested primer 2:CTTGCAGAAAGTCCGACCTC