| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.153656 |
| Chromosome: | chromosome 3 |
| Location: | 8170087 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200350 | SMM12 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 1) PTHR10108//PTHR10108:SF706 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGAGGGGCGAGGGGGTGCATGGCAGCTG |
| Internal bar code: | AACACATCTCCAGGAGACTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 399 |
| LEAP-Seq percent confirming: | 99.789 |
| LEAP-Seq n confirming: | 473 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGAGAAAGAGGCGTTGTG |
| Suggested primer 2: | CAACCAACGGTGTAGCAATG |