| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.153671 |
| Chromosome: | chromosome 6 |
| Location: | 8892483 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g311050 | (1 of 1) K00167 - 2-oxoisovalerate dehydrogenase E1 component beta subunit (BCKDHB, bkdA2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTCCGCCACCGCTGTGTGGCCGGCAGTG |
| Internal bar code: | CCAAAACACGTTTGCGCCGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 772 |
| LEAP-Seq percent confirming: | 85.1064 |
| LEAP-Seq n confirming: | 320 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGATAACAACGGACTTGC |
| Suggested primer 2: | CGCTTGATATCTGCTGTCCA |