Insertion junction: LMJ.RY0402.153694_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.153694
Chromosome:chromosome 6
Location:7414353
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g299700 SOUL1 (1 of 4) IPR006917//IPR011256 - SOUL haem-binding protein // Regulatory factor, effector binding domain; SOUL heme-binding protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGTCGTTCTTCGTGCCCTTCGACATGCA
Internal bar code:GGGAGGTGCTTGGAAAGTAACG

Confirmation - LEAP-Seq

LEAP-Seq distance:1081
LEAP-Seq percent confirming:99.6779
LEAP-Seq n confirming:2476
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CATAGCCAGCTACACAGCCA
Suggested primer 2:AAGAAGGTGAGAGCCAAGCA