| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.153694 |
| Chromosome: | chromosome 6 |
| Location: | 7414358 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g299700 | SOUL1 | (1 of 4) IPR006917//IPR011256 - SOUL haem-binding protein // Regulatory factor, effector binding domain; SOUL heme-binding protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGTCCTCGCAGAATGGGCCCTGCAGGTG |
| Internal bar code: | CTCGAGGATTAGTACTAGCGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 945 |
| LEAP-Seq percent confirming: | 99.7464 |
| LEAP-Seq n confirming: | 1180 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATAGCCAGCTACACAGCCA |
| Suggested primer 2: | AAGAAGGTGAGAGCCAAGCA |