| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.153696 |
| Chromosome: | chromosome 16 |
| Location: | 5342745 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681850 | (1 of 2) 2.4.2.9//2.7.1.48 - Uracil phosphoribosyltransferase / UMP pyrophosphorylase // Uridine kinase / Uridine monophosphokinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGGCGTGCATGTGCCGAGCGCCCGAGCT |
| Internal bar code: | TACTGTCATGGGTAGGGTGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 805 |
| LEAP-Seq percent confirming: | 99.0424 |
| LEAP-Seq n confirming: | 1448 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAACTGTGGAGGAGTGCAG |
| Suggested primer 2: | TGTGTCAGTCGCGAAATAGC |