| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.153700 |
| Chromosome: | chromosome_15 |
| Location: | 283944 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre15.g635600 | NPC1 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAAAGGGGGGAACCACGGGGCTCGCGCGGG |
| Internal bar code: | GGGCCATACTCGGGTGGGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 883 |
| LEAP-Seq percent confirming: | 97.622 |
| LEAP-Seq n confirming: | 1560 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAACTAAAGCTGGCATCGC |
| Suggested primer 2: | TGCACTGCACAGTTGCATTA |