| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.153703 |
| Chromosome: | chromosome 8 |
| Location: | 881257 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g361350 | (1 of 3) PF01936 - NYN domain (NYN) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAGGACATGCAATGGACTTGCATCAGTA |
| Internal bar code: | CTGTGCGCCGGGGCATGTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 764 |
| LEAP-Seq percent confirming: | 97.1494 |
| LEAP-Seq n confirming: | 33331 |
| LEAP-Seq n nonconfirming: | 978 |
| LEAP-Seq n unique pos: | 92 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGCTGTGGAGAGTTGGTG |
| Suggested primer 2: | CGGTGTGTGTGTGTGTGTGT |