| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.153711 |
| Chromosome: | chromosome 2 |
| Location: | 4780699 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g102700 | (1 of 3) PF11735 - Cryptococcal mannosyltransferase 1 (CAP59_mtransfer) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCGACCAGGGGCTGGCAACACAGCAGGC |
| Internal bar code: | CATATACAGGGCTCCGCTTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 309 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 102 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGAGGTACCTTGCACACA |
| Suggested primer 2: | GTACGCGAACTGCGGATTAT |