| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.153717 |
| Chromosome: | chromosome 7 |
| Location: | 6338501 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g357300 | (1 of 1) PTHR33558:SF1 - GLUTAREDOXIN-LIKE PROTEIN C5ORF63 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGGAGGGGGGGGGCAGTCGGACACGTGC |
| Internal bar code: | ATTAGTTCTAATGTATTTCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 422 |
| LEAP-Seq percent confirming: | 99.6427 |
| LEAP-Seq n confirming: | 3625 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATATATGCGCGAACAAAGCC |
| Suggested primer 2: | TGGAGGTGTGTGTGTGTGTG |