| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.153717 |
| Chromosome: | chromosome 7 |
| Location: | 6338537 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g357300 | (1 of 1) PTHR33558:SF1 - GLUTAREDOXIN-LIKE PROTEIN C5ORF63 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACGCCGCCAGCAGTATATGCAGGATGAA |
| Internal bar code: | GGCAGAATCTCATCGTCCGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 165 |
| LEAP-Seq percent confirming: | 98.6928 |
| LEAP-Seq n confirming: | 151 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAGGTGTGTGTGTGTGTG |
| Suggested primer 2: | ATATATGCGCGAACAAAGCC |