| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.153718 |
| Chromosome: | chromosome 2 |
| Location: | 8482488 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g141786 | PDE25 | 3\',5\'-cyclic-nucleotide phosphodiesterase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTGCCGGAGCGGTCAAAGCACTAGCAA |
| Internal bar code: | CCCAATTCTCCGCTTCCCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 175 |
| LEAP-Seq percent confirming: | 61.8939 |
| LEAP-Seq n confirming: | 817 |
| LEAP-Seq n nonconfirming: | 503 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCATGGTCAAGAAACGGT |
| Suggested primer 2: | CCGTAAGCAGACAGCAAACA |