| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.153719 |
| Chromosome: | chromosome 12 |
| Location: | 561994 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492750 | (1 of 5) 3.2.1.1 - Alpha-amylase / Glycogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCATGGGCATGCACGCGCAGGTGGCGGTG |
| Internal bar code: | GTAAGTAAGTACGTGGGGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 676 |
| LEAP-Seq percent confirming: | 84.8837 |
| LEAP-Seq n confirming: | 146 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCGCCTTTGACCATGTTT |
| Suggested primer 2: | TCGTACTGCTGCTTGGTGTC |