| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.153730 |
| Chromosome: | chromosome 10 |
| Location: | 2479045 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g436100 | MLK1 | Mixed lineage protein kinase; (1 of 2) PF00069//PF01590//PF07714 - Protein kinase domain (Pkinase) // GAF domain (GAF) // Protein tyrosine kinase (Pkinase_Tyr) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGCGGGTGTGCTGTCTGACTGGCCACG |
| Internal bar code: | TCCCTTTTCGCTCGGGGGAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1001 |
| LEAP-Seq percent confirming: | 98.5841 |
| LEAP-Seq n confirming: | 2228 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTTCGATAGCAGCGGAAG |
| Suggested primer 2: | GTTACAGAACCTGAGGGGCA |