| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.153745 |
| Chromosome: | chromosome 3 |
| Location: | 4588579 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176961 | (1 of 37) IPR009030 - Insulin-like growth factor binding protein, N-terminal | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATCATCCGCTGCCCCCGCTGGCCGCATC |
| Internal bar code: | CTTTAGCGGCGAGCCGTTGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 501 |
| LEAP-Seq percent confirming: | 99.8534 |
| LEAP-Seq n confirming: | 681 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGTTCCACACCGAACAC |
| Suggested primer 2: | TACTGCTGCCACTGCTGCTA |