Insertion junction: LMJ.RY0402.153750_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.153750
Chromosome:chromosome 1
Location:6952690
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g050150 NFO1 NADH:flavin oxidoreductase/NADH oxidase; (1 of 1) 1.6.99.1 - NADPH dehydrogenase / NADPH diaphorase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTAATCGCTGCACCGCGACCGGCCGCTTC
Internal bar code:AGCAGTGTTCAGGGTCGGGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1001
LEAP-Seq percent confirming:96.1876
LEAP-Seq n confirming:21673
LEAP-Seq n nonconfirming:859
LEAP-Seq n unique pos:77

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTCTGTTATCTCGGCTGC
Suggested primer 2:TGGTCACCACTACCTCCACA