Insertion junction: LMJ.RY0402.153753_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.153753
Chromosome:chromosome 6
Location:5556371
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g285300 SUT1 Sugar transporter; (1 of 1) K08200 - MFS transporter, OCT family, solute carrier family 22 (organic cation transporter), member 3 (SLC22A3, OCT3) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTGTTGCTGCAGCTGCCCGAGACGCGCGG
Internal bar code:GCGCGGCGAGGATAAGGCGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1016
LEAP-Seq percent confirming:99.9038
LEAP-Seq n confirming:2078
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGCTCATACAAGCACCAA
Suggested primer 2:ACACGGAATCCACCGTAGAG