Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.153757 |
Chromosome: | chromosome 10 |
Location: | 2548349 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g436800 | (1 of 2) PF01833//PF07691//PF10162 - IPT/TIG domain (TIG) // PA14 domain (PA14) // G8 domain (G8) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGGCCAGCACAGCTACAGTCCAGCTCAG |
Internal bar code: | AATGCGACGCCGCTGCCCTCCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1132 |
LEAP-Seq percent confirming: | 99.6328 |
LEAP-Seq n confirming: | 814 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATCCTCGACGAAGACACACC |
Suggested primer 2: | CCACATTCGAGCTCAACAGA |