| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.153765 |
| Chromosome: | chromosome 14 |
| Location: | 1821145 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g620200 | (1 of 1) IPR011527//IPR021261 - ABC transporter type 1, transmembrane domain // Protein of unknown function DUF2838 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGGGCGAAAAATAGCAACGCAATTAGTC |
| Internal bar code: | ATCGCACAGGCGTGGGAGTCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 263 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 664 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTACTAGTGGAGAGCCCCC |
| Suggested primer 2: | GAATCACTGGGAGACGGTGT |