| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.153799 |
| Chromosome: | chromosome 13 |
| Location: | 4408372 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g603176 | (1 of 2) PTHR23023:SF4 - FLAVIN-CONTAINING MONOOXYGENASE | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTGCATATTCCACATTACATGGTCACA |
| Internal bar code: | ACGAGCTCCGTATGTTTGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 159 |
| LEAP-Seq percent confirming: | 99.811 |
| LEAP-Seq n confirming: | 1584 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCAGACTGGTCCGTAACC |
| Suggested primer 2: | ACCTCCACAGCCACCTACAC |