Insertion junction: LMJ.RY0402.153816_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.153816
Chromosome:chromosome 12
Location:7745696
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g553850 TUL1 (1 of 781) IPR000104 - Antifreeze protein, type I; Tubulin-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCGCTTGAGCTTCTCGTCAAGAGCGCTG
Internal bar code:CATGGGTGCCCGGGGGGGAATA

Confirmation - LEAP-Seq

LEAP-Seq distance:445
LEAP-Seq percent confirming:99.3068
LEAP-Seq n confirming:573
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AAAATGTGCTGATGCTGCTG
Suggested primer 2:CCTTGTTGTGGCAGGGTACT