| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.153914 |
| Chromosome: | chromosome 3 |
| Location: | 4015669 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172150 | (1 of 1) PTHR10663//PTHR10663:SF182 - GUANYL-NUCLEOTIDE EXCHANGE FACTOR // SH2 DOMAIN-CONTAINING PROTEIN 5 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCCTGCACACCTAGCGTCCGCAGTCAT |
| Internal bar code: | GGCTCTCTTTTCGGCTATGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 585 |
| LEAP-Seq percent confirming: | 86.8421 |
| LEAP-Seq n confirming: | 99 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTTCCTGGAGGAGGTGCT |
| Suggested primer 2: | AGAAGCCCCGGTACAGTCTT |