| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.153916 |
| Chromosome: | chromosome 7 |
| Location: | 5558637 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g351500 | (1 of 1) K15078 - structure-specific endonuclease subunit SLX1 (SLX1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCTTGGGGCTCCGGAGCCGCTTGGACA |
| Internal bar code: | GAGGAACCCGCAATGTGAGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 148 |
| LEAP-Seq percent confirming: | 99.3421 |
| LEAP-Seq n confirming: | 302 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGCCTGCAGCTAAAAACC |
| Suggested primer 2: | TAACCCAGACCAGGAAGGTG |