| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.153938 |
| Chromosome: | chromosome 1 |
| Location: | 1740226 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g009325 | (1 of 2) 1.14.11.18 - Phytanoyl-CoA dioxygenase / Phytanoyl-CoA hydroxylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTGGGTCCGGAGGCGTGGAGCAGGGTG |
| Internal bar code: | GCGATGATGGTCATGCCTCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 789 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 443 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGTTGGGTGACGTGACAG |
| Suggested primer 2: | CAGGGTATTGTGCAAGCTGA |