| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.153942 |
| Chromosome: | chromosome 10 |
| Location: | 407870 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g420600 | (1 of 2) PTHR22840//PTHR22840:SF12 - FAMILY NOT NAMED // WD REPEAT-CONTAINING PROTEIN 36 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGGCCAGTTGGCGCCGTACGACCTGGC |
| Internal bar code: | GTACGGAAGGGAGGGTCAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 924 |
| LEAP-Seq percent confirming: | 94.5613 |
| LEAP-Seq n confirming: | 5529 |
| LEAP-Seq n nonconfirming: | 318 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGCTACACCGTGCAGTT |
| Suggested primer 2: | ATGTGCGTGACGAATTGTGT |