| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.153988 |
| Chromosome: | chromosome_9 |
| Location: | 4735910 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g397142 | TRP15 | Transient receptor potential ion channel protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGGAGTGGTGATTGGCAACCCCCATCCCA |
| Internal bar code: | TATACTTGTTGCGCCGGCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 711 |
| LEAP-Seq percent confirming: | 61.5942 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCCGTCCTCCACACAAAC |
| Suggested primer 2: | CGGTGTGTGTGTGTGTGTGT |