| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.154027 |
| Chromosome: | chromosome_16 |
| Location: | 2092208 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g657650 | FAP35 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCTTGGGGTTGGGACTAGGACCAGAGCTT |
| Internal bar code: | GGATCACCAAAAGCAGTGTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 828 |
| LEAP-Seq percent confirming: | 99.0937 |
| LEAP-Seq n confirming: | 656 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACCTAATTGCATGCAGGG |
| Suggested primer 2: | TGCATCGTGAGTGGGAAATA |