| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.154032 |
| Chromosome: | chromosome 12 |
| Location: | 4057156 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g517550 | RRM10 | Putative ribosomal RNA methyltransferase; (1 of 2) 2.1.1.137 - Arsenite methyltransferase / S-adenosyl-L-methionine:methylarsonite As-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACTACCAGCCTACCAGCCCGGACGTCCC |
| Internal bar code: | TATTGTGGGCAGGTTGCTATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 496 |
| LEAP-Seq percent confirming: | 99.1228 |
| LEAP-Seq n confirming: | 452 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATATTGCAAGCCCTGTGTCC |
| Suggested primer 2: | GAGTGAGTGAGTGGTGGGGT |