| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.154061 |
| Chromosome: | chromosome 3 |
| Location: | 5745598 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g188250 | AGP4,STA6 | ADP-glucose pyrophosphorylase small subunit; (1 of 1) PTHR22572//PTHR22572:SF79 - SUGAR-1-PHOSPHATE GUANYL TRANSFERASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACATTTCGGCAGCTGCCGCAGCGAGGAG |
| Internal bar code: | TCCGTCTAGTCAATGGTCGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 864 |
| LEAP-Seq percent confirming: | 88.6878 |
| LEAP-Seq n confirming: | 1960 |
| LEAP-Seq n nonconfirming: | 250 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGCGCAGTTCTCGTTCTA |
| Suggested primer 2: | AAGAGCCCTCTCTACCTCCG |