| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.154073 |
| Chromosome: | chromosome 4 |
| Location: | 215148 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217300 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATCTGACATAAACTAAGGAGTTCTGAGG |
| Internal bar code: | GGCGCAGCCGGGTAAAGGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 540 |
| LEAP-Seq percent confirming: | 97.8873 |
| LEAP-Seq n confirming: | 139 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGTGATTGCTGACTTCTG |
| Suggested primer 2: | AGCCTCCTGTCGCATTTCTA |