| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.154117 |
| Chromosome: | chromosome 1 |
| Location: | 2072282 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g011100 | CAS1 | (1 of 1) 5.4.99.8 - Cycloartenol synthase / 2,3-epoxysqualene--cycloartenol cyclase; Terpenoid cylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTTGTTGGGAATGGCGCCGTTGGGAGT |
| Internal bar code: | ACGTACCGGGGGGCACACGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 223 |
| LEAP-Seq percent confirming: | 99.497 |
| LEAP-Seq n confirming: | 989 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTTAACACAGCGCCACAG |
| Suggested primer 2: | CTCCTCCCCTACCGTCTACC |