| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.154131 |
| Chromosome: | chromosome 10 |
| Location: | 683218 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422350 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAGGGAAGTCATGCATGTTCGTAGGGAA |
| Internal bar code: | GCGGTTTCGGCTTGGCCTCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1070 |
| LEAP-Seq percent confirming: | 99.8024 |
| LEAP-Seq n confirming: | 505 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACCGAGCGTAACTTTGT |
| Suggested primer 2: | CACGCAGCAATCAGTTTTGT |