| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.154138 |
| Chromosome: | chromosome 1 |
| Location: | 4610996 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g031700 | FKM1 | DNA methylase in the FkbM family; (1 of 13) PF05050 - Methyltransferase FkbM domain (Methyltransf_21) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTTCCTGCTTGACCTCGTGGTCGTATAT |
| Internal bar code: | GCCTCGGGAACTGTGGCGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 198 |
| LEAP-Seq percent confirming: | 21.5232 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 237 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCAAACTAAACCTAGCCC |
| Suggested primer 2: | GAATTAGGCGTGCTGGGTAA |