| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.154214 |
| Chromosome: | chromosome 9 |
| Location: | 2994156 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387550 | CGL142 | Conserved in the Green Lineage | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGACCGACCACCACTGCACGACCTTACT |
| Internal bar code: | ACCAAATTGTGATGTATGTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 578 |
| LEAP-Seq percent confirming: | 99.4695 |
| LEAP-Seq n confirming: | 1125 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTTCCGAGGGTACCACAG |
| Suggested primer 2: | AATACCCAATTTGCGTGCTC |