Insertion junction: LMJ.RY0402.154246_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.154246
Chromosome:chromosome 17
Location:2337922
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGAGCAGTGCGCTTCTGTGCGCGGCAAG
Internal bar code:TAAGTGGTCAGTAAATTCGCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:245
LEAP-Seq percent confirming:99.5098
LEAP-Seq n confirming:203
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCTAACGGAATTGTTCGCAT
Suggested primer 2:GCATTGTGAACAGGGGAAGT