| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.154262 |
| Chromosome: | chromosome 16 |
| Location: | 4672164 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687300 | CPLD61 | Conserved in the Plant Lineage and Diatoms; (1 of 3) PF13599 - Pentapeptide repeats (9 copies) (Pentapeptide_4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTCCCTCCACCAGCCAGCCTGTTGTCAC |
| Internal bar code: | TCGTATAGTACACCTCGCGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 437 |
| LEAP-Seq percent confirming: | 99.1786 |
| LEAP-Seq n confirming: | 483 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGTTCACCTGGGTGTGTG |
| Suggested primer 2: | AAGCAGCAGCAGTTGCAGTA |