| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.154273 |
| Chromosome: | chromosome 6 |
| Location: | 1884320 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g263200 | FAP325 | Flagellar Associated Protein 325 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCGTGGGTACATTGCTGCTGTGGCTGC |
| Internal bar code: | GCGCCCTCACGGCACCTCATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 933 |
| LEAP-Seq percent confirming: | 99.7858 |
| LEAP-Seq n confirming: | 4192 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGCACACAGTTCACGCAC |
| Suggested primer 2: | AAACGTCGTAACCTGCCATC |