| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.154274 |
| Chromosome: | chromosome 6 |
| Location: | 6874234 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g295500 | (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGGAGGCTCCGGTATCCGGCAGTGGCA |
| Internal bar code: | TAAATTTCAAAGCAGTTGTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 470 |
| LEAP-Seq percent confirming: | 99.1766 |
| LEAP-Seq n confirming: | 2650 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTCTGGCCTCTGTTCTG |
| Suggested primer 2: | TTGGAGCCCATACGTACACA |